The first step in the biosynthesis of griseofulvin by P. griseofulvin is the synthesis of the 14-carbon poly-β-keto chain by a type I iterative polyketide synthase (PKS) via iterative addition of 6 malonyl-CoA to an acyl-CoA starter unit. The 14-carbon poly-β-keto chain undergoes cyclization/aromatization, using cyclase/aromatase, respectively, through a Claisen and aldol condensation to form the benzophenone intermediate. The benzophenone intermediate is then methylated via S -adenosyl methionine (SAM) twice to yield griseophenone C. The griseophenone C is then halogenated at the activated site ortho to the phenol group on the left aromatic ring to form griseophenone B. The halogenated species then undergoes a single phenolic oxidation in both rings forming the two oxygen diradical species. The right oxygen radical shifts alpha to the carbonyl via resonance allowing for a stereospecific radical coupling by the oxygen radical on the left ring forming a tetrahydrofuranone species.  The newly formed grisan skeleton with a spiro center is then O -methylated by SAM to generate dehydrogriseofulvin. Ultimately, a stereoselective reduction of the olefin on dehydrogriseofulvin by NADPH affords griseofulvin.  
Fungi will often cause the area around the nail to become red and irritated. Initially a fungus will take hold at the base or edges of the nail. Once the infection continues to spread you'll notice changes in your nail and nail bed. You may experience mild discomfort, itching, or perhaps even sharp pain around the cuticle which is the skin surrounding the nail. Bleeding may begin to occur and often detachment from the cuticle to the nail may result. The nail will begin a slow transformation to perhaps a yellow, brown, or white coloration. Nails will often become thicker and extremely brittle. You may experience discomfort when you put weight on any fungal infected nail.
In contrast to the azoles and other antimycotic drugs, the mechanism of action of ciclopirox is poorly understood.  However, loss of function of certain catalase and peroxidase enzymes has been implicated as the mechanism of action, as well as various other components of cellular metabolism. In a study conducted to further elucidate ciclopirox's mechanism, several Saccharomyces cerevisiae mutants were screened and tested. Results from interpretation of the effects of both the drug treatment and mutation suggested that ciclopirox may exert its effect by disrupting DNA repair, cell division signals and structures (mitotic spindles) as well as some elements of intracellular transport.  It acts by inhibiting the membrane transfer system by interrupting the Na + K + ATPase .  It is currently being investigated as an alternative treatment to ketoconazole for seborrhoeic dermatitis as it suppresses growth of the yeast Malassezia furfur . Initial results show similar efficacy to ketoconazole with a relative increase in subjective symptom relief due to its inherent anti-inflammatory properties.